Journal: PLOS Pathogens
Article Title: Chimeric PRR T-cell-engager targeting cell surface β-1,3-glucan for invasive candidiasis
doi: 10.1371/journal.ppat.1013508
Figure Lengend Snippet: (A) Affinities of anti-hCD3 variants to Jurkat cells. MFI of Jurkat cells incubated with antibodies were determined by flow cytometry. Data are means ± SD from triplicates of one representative experiment of three. (B) BLI analysis for interaction between hXJ104 and huCD3D&CD3E protein. KD, ka and kids values showed in the table. (C-E) Flow cytometry for hXJ104 binding to Jurkat cells (C), splenocytes from huCD3ε transgenic mice (D), and various clinically prevalent Candida species (E); Data are representative of three independent experiments (B, C, D, E); MFI, mean fluorescence intensity; BLI, bio-layer interferometry; KD, equilibrium dissociation constant; ka, association rate values; kd, dissociation rate values.
Article Snippet: Recombinant Human CD3D & CD3E Heterodimer Protein (ECD, His Tag) and Recombinant Mouse CD3D & CD3E Heterodimer Protein (Flag & His Tag) were purchased from SinoBiological.
Techniques: Incubation, Flow Cytometry, Binding Assay, Transgenic Assay, Fluorescence